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Article in Spanish | LILACS | ID: lil-724856

ABSTRACT

OBJETIVO: El propósito de este estudio fue determinar la presencia de genes de virulencia en cepas de Streptococcus mutans (S. mutans) aisladas desde saliva de individuos de diferentes edades y asociarlas con el índice COPD y ceod según corresponda. MATERIAL Y MÉTODO: A partir de un total de 120 individuos de ambos sexos, se conformaron 4 grupos de 30 personas, que se separaron de acuerdo con los siguientes rangos etarios: 3-5, 6-9, 12-15 y mayores de 18 años. A cada individuo se le determinó el índice COPD y ceod según correspondiera y se realizó recuento salival de S. mutans. La detección de los genes de virulencia: gtfB y spaP se realizó por reacción en cadena de la polimerasa convencional. RESULTADOS: Se estableció una asociación positiva entre el recuento bacteriano e índice COPD y ceod. El 100% de las cepas aisladas evidenciaron la presencia del gen gtfB y el 63,6% presentaron el gen spaP. No hubo evidencia estadísticamente significativa que relacionará un alto recuento bacteriano e índice COPD y ceod con la mayor presencia de genes que codifican factores de virulencia en cepas de S. mutans


AIM: The aim of this study was to determine the presence of gtfB and spaP virulence genes in Streptococcus mutans strains isolated from saliva taken from individuals of different ages. MATERIAL AND METHOD: A total of 120 individuals of both sexes were studied. They were assigned to one of 4 groups, with 30 individuals in each one, according to age; 3-5, 6-9, 12-15, and older than 18 years old. DMFT (decayed, missing, and filled teeth) and DMFT indexes were determined in each participant, depending on his/her age. S. mutans microbial counts were performed. The gtfB and spaP virulence genes were detected using conventional PCR. RESULTS: A positive association was found between microbial count and DMFT and DMFT indexes. All the isolated strains demonstrated the presence of gtfB, and 63.6% of the strains had spaP genes. No association was found between high bacterial counts or DMFT and DMFT indexes with the presence of genes that code for virulence factors in S. mutans strains


Subject(s)
Humans , Male , Female , Child, Preschool , Child , Adolescent , Adult , Middle Aged , Aged , Aged, 80 and over , Young Adult , Saliva , Streptococcus mutans , Streptococcus mutans/genetics , Streptococcus mutans/pathogenicity , Virulence , DMF Index , Dental Caries/epidemiology , Saliva , Polymerase Chain Reaction , Risk Assessment , Dental Caries/microbiology , Bacterial Load
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